Eremeeva, T. E.; Bykova, T. O. Masuelli, Martin Alberto. 2003;89(2):31120. where tr is the column retention time in minutes (2 of 0.9792). Transdermal drug delivery is an attractive alternative that addresses the limitations of oral and parenteral routes of drug administration; it enables controlled release and long-term systemic drug delivery through the skin, avoids hepatic first-pass effect, avoids gastrointestinal drug degradation, reduces discomfort and trauma resulting from hypodermic injections, and prevents safety hazards associated with dangerous medical waste from needles [1, 2]. Chromatographic characterization of polymers: Hyphenated and multidimensional techniques. Each skin pores was captured using a microscope and the obtained images were analyzed using ImageJ software (National Institutes of Health, Bethesda, USA). The micropores of the human cadaver skin were regenerated according to the time. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. GPC Symp. Intrinsic viscosity-molecular weight relationship for chitosan. 2015;23(3):2239. Charge: Anionic. Epub 2012 May 28. Rheology of dextran solutions. FITC-dextran solution (600L) was added to the donor chamber. Ther. The Mark-Houwink parameters and the effect of temperature on them are specifically studied. WebThomas Gutberlet works at Jlich Centre for Neutron Science (JCNS-2), Forsch and is well known for Lipoteichoic Acid, Neutron Reflectometry and Neutron Reflectivity. Hindered diffusion of dextran and ficoll in microporous membranes. By studying the molecular weights of various solutions of polymer, Solomon-Ciuta arrived at the formula which allowed the calculation of the intrinsic viscosity of polymer solutions by a single viscosity determination. of different origins. Whiteside PJD, Chininis JA, Schellenberg MW, Qian C, Hunt HK. Polysaccharides-based nanoparticles as drug delivery systems. Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. ; van Nostrum, C.F. Curley SA. Int. (1954). After the pore closure examination, each sample was stored in a 10% neutral buffered formalin awaiting haematoxylin/eosin (H&E) staining. In general, Mv is not experimentally accessible, whereas other average molecular weights are accessible. J. Braz. Here we have the hydrodynamic radius estimate for the 3kDa dextran and 1.5 kDa dextran by the Zetasizer calculator, thanks to Alan. Alan F Rawle, t J Pharm Sci. Lee WR, Shen SC, Sun CK, Aljuffali IA, Suen SY, Lin YK, Wang JJ, Fang JY. FITC-dextran solution was prepared at 1mM in PBS. Human skins (Full-thickness human skin) were supplied by HansBiomed. WebDextran 40 Pharmaceutical Quality. The measurements of the transepidermal water loss (TEWL) were taken using the Tewameter (Courage-Khazaka Electronic GmbH, Cologne, Germany) to evaluate the skin barrier function after microporation. The hydrodynamic radius of 2-NBDG glucose was too small to measure. ; Weisz, J. (2008). WebDextran displays excellent biocompatibility and have been used clinically for over 50 years. After 8h, the micropore became more distorted from the previous circle, suggesting that the micropores were closing. Smith, David R.; Raymonda, John W. (1972). 8. Kasaai, Mohammad R. (2007). 4h after microporation, the sizes of the micropore decreased slightly. The Mark-Houwink a constant is close to 0.5 in poor solvents. Carbohydrate Polymers 72, 356-360. Barshtein, G.; Tamir, I.; Yedgar, S. (1998). 2004;25(9):155967. Journal of Chemical Education, 49(8), 577-579. We believe that the microporation device can be used as a potential technique for effective transdermal drug delivery. Advances in chemistry series Washington, DC: American Chemical Society., pp. The molecular weight of dextran samples can be obtained by a variety of methods including light scattering, HPLC/SEC, sedimentation, osmometry and end-group analysis. The flattening of the curves at higher molecular weight is in agreement with an increased branching density. Pharmaceutical Quality Dextran 40 is available from Pharmacosmos as active pharmaceutical ingredients (API) as excipient for final formulation, drug product, for medical devices, for production of solutions for injection and infusion and much more. An undergraduate physical chemistry experiment. This method utilizes promising strategies that have made a significant impact in transdermal delivery [6], they include: iontophoresis, microneedles, sonophoresis, radiofrequency (RF), and chemical enhancers [7,8,9,10,11]. Kasaai, M. R.; Arul, J.; Charlet, G. (2000). Thus, it is a common practice to express the frictional coefficient of rigid structures as. Epub 2022 Feb 2. FOIA WebDextran produced from Weissella cibaria JAG8 was purified and characterized. The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. Formation of dextran hydrogels by crystallization. SWC designed and coordinated the research. In this study, we investigated the effectiveness of RF microporation technology in vitro, by evaluating the permeation of fluorescent-conjugated dextran (FITC-dextran) of various molecular weights through the human and pig skin after microporation, using a transdermal diffusion cell system. The [] is connected to the dimensions of the biopolymer molecule isolated in a certain solvent. WebFluorescein isothiocyanatedextran average mol wt 60,000-76,000; CAS Number: 60842-46-8; Synonyms: FITCDextran; find Sigma-Aldrich-FD70S MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich The values of 0 decreases from 2.5429 to 2.3326 x 1023mol-1 demonstrating a relative flexibility of particles. The above data are not useful because it can not be compared with those obtained in this work. Dextran Triple Detector Application Note 1, Viscotek GmbH, USA. However, in vitro investigations are very few. There is a deviation from linearity after 580kDa [69, 70]. Effect of temperature on the intrinsic viscosity and conformation of chitosan in dilute HCl solution. For data requests, please contact the authors. Synthesis of water-soluble and water-insoluble amphiphilic derivatives of dextran in organic medium. In a poor solvent which shows a positive heat of mixing, segments of a polymer molecule attract each other in solution more strongly than attract the surrounding solvent molecules. It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free In contrast to linear chains, power law behavior is observed only asymptotically at large Mv. Journal of Polymer Science, 17, 527-546. Dextran; Weissella cibaria; exopolysaccharide; prebiotic; skimmed milk. Micropores are produced by the arrangement of microelectrodes on the skin at precise dimensions, then RF energy generated by alternating currents induces ionic vibrations between electrodes with positive or negative charges. government site. Huggins equation [41] gives the relation to the relative viscosity increase (i). Challenges for the modern analytical ultracentrifuge analysis of polysaccharides. Covis, Rudy; Ladaviere, Catherine; Desbrieres, Jacques; Marie, Emmanuelle; Durand, Alain. The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC dextran measured in plasma 1 h after its oral application is a marker of small intestinal permeability. In Vitro Evaluation of Postbiotics Produced from Bacterial Isolates Obtained from Rainbow Trout and Nile Tilapia against the Pathogens, Dextran-Mimetic Quantum Dots for Multimodal Macrophage Imaging, Isolation of lactic acid bacteria starters from, Characterization of Antibacterial Cell-Free Supernatant from Oral Care Probiotic. Fractional thermolysis by bipolar radiofrequency facilitates cutaneous delivery of peptide and siRNA with minor loss of barrier function. 4K and M.W. Sci. Guner, Ali (1999). ACS Nano. ; Picout, D.R. Corp. (Seoul, Korea). However, when molecules deviate from globular to non-spherical or elongated structures then Rg/Rh tend to values upwards of Pore closure examination was performed using an optical microscope (BX43, Olympus, Japan) immediately (time 0), 4, 8, and 24h following device application. Hydrodynamic Properties of Tragacanthin. Harding, S. E.; Varum, K.; Stoke B.; Smidsrod, O. Granath, K. A. All authors read and approved the final manuscript. Biodegradable dextran hydrogels crosslinked by stereocomplex formation for the controlled release of pharmaceutical proteins. Pharm Res. The regeneration of micropores in the human cadaver skin caused by microporation was confirmed using an optical microscope and haematoxylin/eosin (H&E) staining. The importance of this type of study lies in the analysis of the polysaccharide behavior at industrial processes, the requirements to reduce energy, avoid flow problems and product quality control. Eng. The range of temperatures used was 25-50 (0.1) C and it was regulated by thermostatic bath Haake C. The viscosity measurements required Hagenbach-Couette correction time (tg 37.31s). Webdextran hydrodynamic radius. Kirkland, J. J.; Dilks, C. H.; Rementer, S. W. (1992). Herwadkar A, Banga AK. @ Nilimesh See attached. (1991). Format). Intrinsic viscosities of polyelectrolytes in the absence and in the presence of extra salt: Consequences of the stepwise conversion of dextran into a polycation. 24h after microporation, the pore sizes reduced from 64.8817.20m (0h) to 48.854.27m (24h), and the pore depths reduced from 83.9120.56m (0h) to 63.9527.61m (24h). Pig skins (Micropig Franz cell membrane) were supplied by Medi Kinetics Co., Ltd. (Pyeongtaek, Korea). Harding, Stephen E. (1997). Viscotek GmbH, Europe. H&E stained images were analyzed using Image J software. (2013). 2019;9(3-S):9726. Recent developments in polysaccharide-based materials used as adsorbents in wastewater treatment. II. Macromolecules. 151-166. Pharmacol Pharm. Sintov AC, Krymberk I, Daniel D, Hannan T, Sohn Z, Levin G. Radiofrequency-driven skin microchanneling as a new way for electrically assisted transdermal delivery of hydrophilic drugs. statement and Hydrodynamic properties, such as the intrinsic viscosity, [], and equilibrium solution properties such as the hydrodynamic radius RH can be combined to construct dimensionless quantities that are universal since they are of being independent of the macromolecular particle size, while they depend more or less sensitively on its shape [51]. Hydrodynamic cavitation (HC), a common phenomenon that can produce local excessive temperature, high pressure and large amount of energy. Provided by the Springer Nature SharedIt content-sharing initiative. 7 8 Carbohydrate Polymers 88 1440-1444. For rigid macromolecules, it is also conventional to combine a solution property with the volume of the particle itself, or with a quantity directly derived from it [54, 55, 56]. Based on these results a difference of approximately 1015% between the amounts of drugs permeated through the human skin and pig skin models should be considered during human clinical trials. Quintanilla-Pineda M, Achou CG, Daz J, Gutirrez-Falcon A, Bravo M, Herrera-Muoz JI, Pea-Navarro N, Alvarado C, Ibaez FC, Marzo F. Foods. Fusco V, Quero GM, Cho GS, Kabisch J, Meske D, Neve H, Bockelmann W, Franz CM. In vivo iontophoretic delivery of salmon calcitonin across microporated skin. Pure Appl. Figure 2 shows the classical method for calculating Mark-Houwink parameters, where a linear relation between the intrinsic viscosity and the molecular weight is established for each temperature. 2012;87(6):1728. Pathan IB, Setty CM. Figure5 shows the cumulative amount of the FITC-dextran (M.W. Analytical Chemistry. At 20C data "a" value obtained in this work is intermediate to those given by references [1, 37] while the value of kM-H is lower. Molecular Weight Distributions of Water-Soluble Polymers by Flow Field-Flow Fractionation. Biological skin substitutes for wound cover and closure. Progress in Polymer Science, 30, 38-70. In vitro studies with human cadaver skin is a viable, ethical, and economical alternative for in vivo animal studies. We observe that this maximum radius is below Copyright 2006-2023 Thermo Fisher Scientific Inc. All rights reserved, Dextran, Tetramethylrhodamine, 2,000,000 MW, Lysine Fixable, Sulen und Kartuschen fr die Chromatographie, Kunststoffartikel und Zubehr fr das Labor, Spektroskopie, Element- und Isotopenanalyse, Alle Themen fr Hilfe und Support anzeigen, Status und Nachverfolgung von Bestellungen, Reagenzien und Kits fr die Neurowissenschaft, Discover how to track and trace cells by imaging , Hier finden Sie unsere brigen Tracking-Produkte fr Sugetier-Zellkulturen, Molecular Probes Dextran-Konjugate Tabelle14.4. Normally, the skin loses very little water loss whereas damaged skin loses more [22]. RBECs were passaged onto cell culture inserts as described and cultured for a further 714 days in vitro. 2021 Aug;13(4):915-925. doi: 10.1007/s12602-021-09751-1. 2018 Jan;184(1):1-11. doi: 10.1007/s12010-017-2522-4. International Journal of Biological Macromolecules, 42, 133-137. Senti, F. R.; Hellman, N. N.; Ludwig, G.; Babcock, E.; Tobin, R.; Class, C. A., (1955). It is worth noting that the size of macromolecules is much bigger than that of solvent (water) molecules [50]. Radiofrequency with microneedling. ; Fran, H.P. ; Wood, P.J. ; Veldhuis, T.F.J. Molecular weight (M v) and hydrodynamic properties can be calculated from there. Carbohydr Polym. Optical properties of dextran in solution and in films. The intrinsic viscosity calculation requires several concentrations, and because of this inconvenient will not use the Huggins method in this paper. Liu Zonghua; Yanpeng Jiao; Yifei Wang; Changren Zhou; Ziyong Zhang (2008). These constants can be determined experimentally by measuring the intrinsic viscosity of several polymer samples for which the molecular weight has been determined by an independent method (i.e. The characteristic Rg/Rh value for a globular protein is ~0.775, which means that Rg is smaller than Rh. Harding, Stephen E. (2005). The hydrodynamic radius is important in predicting transretinal penetration Carroll PJ. (1999). volume24, Articlenumber:22 (2020) Materiaux 1-5. Fixable: Fixable via Lysine. Springer Nature. Therefore, it is suitable as model molecules with different molecular weights for transdermal drug delivery [29]. It is well tolerated on parenteral infusion by most people but a very low incidence Int J Food Sci Nutr. Mark-Houwink-Kuhn-Sakurada Exponent at the Condition. European Polymer Journal, 43, 1744-1753. Mainaa, Ndegwa Henry; Pitknen, Leena; Heikkinen, Sami; Tuomainen; Pivi; Virkki, Liisa; Tenkanen, Maija (2014). The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. CAS and transmitted securely. Determination of intrinsic viscosities of macromolecules and nanoparticles. The skin was treated with the RF-based microporation device, inducing micropore ablation through alternating electrical current. Bose, A.; Rollings, J. E.; Caruthers, J. M.; Okos, M. R.; Tsao, T. G. (1982). The increased TEWL values which were proportional to the number of microspores indicate SC disruption and formation of microchannels in the skin. Teixeira CG, Fusieger A, Milio GL, Martins E, Drider D, Nero LA, de Carvalho AF. 4K, b M.W. ; Ortega, A.; Garcia de la Torre, J.; Harding, S.E. Markiertes Dextran wird hufig per Mikroinjektion in die Zellen eingebracht. The analysis of errors using the Solomon-Ciuta method is compared with HM [59]. The parameters of Mark-Houwink for polymers can vary with the polymer solution and temperature. Microporation was performed 2, 5, and 10 times on each sample. where c is the solute concentration (g/cm3), [] is the intrinsic viscosity (cm3/g), and kH represents Huggins constants (cm3/g). WebLabeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the The author thanks Universidad Nacional de San Luis (Project 2-81/11), FONCyT (PICT 2004-N23-2548 and PICT 2008-N21-84), and CONICET (PIP 6324 Res. Kuge Takashi; Kimie Kobayashi; Shinichi Kitamura; Hiroshi Tanahashi. The first term on the left-hand side of equation 2 (i/c) has a linear relation as a function of the concentration (c in g/cm3), where is the intrinsic viscosity [] is given by (c0) [42, 43]. The same way, many of these authors emphasize the non-linear or hyperbranched of dextran molecular weight range from 9 to 2000kDa [3,5,8,16, 37,38]. 4K was 10.611.68 and 13.440.18M in human and pig skin, respectively. The correct way to interpret what Mark-Houwink parameters is determined graphically ln [] versus ln Mw, where Mw is the molecular weight provided by manufacturer, and calculate the percentage relative error (%RE) respect to Mw. (2003). Experimental Review on Intrinsic Viscosity Measurements and Temperature Effect. The mostly used commercial product/ polysaccharide is dextran, and it is produced from bacterium Leuconostoc mesenteroides, with an overall degree of branching of about 5% [1-3][1]. Kano S, Todo H, Furui K, Kenichi S, Tokudome Y, Hashimoto F, Kojima H, Sugibayashi K. Comparison of several reconstructed cultured human skin models by microscopic observation: their usefulness as an alternative membrane for skin in drug permeation experiments. This device can generate microchannels from the SC through to the outer dermis and allow transdermal delivery of active agents. 1905/05) for their financial support. A sugar cane native dextran as an innovative functional excipient for the development of pharmaceutical tablets. The equation 4 can be rearranged and resulted in a modified M-H equation as follows: where the value qM-H is the polydispersity correction factor, and it is a statistical function of the molecular weight distribution. The calculated dextran molecular weights are corroborated by those tabulated data by the manufacturer with a relative error lower than 10%, being the exception dextrans 580 and 2000kDa with the highest relative error. This conjecture found to be correct. Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. Citation-(RIS Bookshelf Exp Cell Res. Boudry I, Blanck O, Cruz C, Blanck M, Vallet V, Bazire A, Capt A, Josse D, Lallement G. Percutaneous penetration and absorption of parathion using human and pig skin models in vitro and human skin grafted onto nude mouse skin model in vivo. This result could reflect the presence of some aggregated species within the lysozyme sample solution. Table1 shows TEWL values measured immediately after the microporation of human and pig skins. To improve drug permeability, micropore ablation using RF is performed by passing an alternating electrical current through the skin at a frequency higher than 100kHz. Viscosity, sedimentation, and light-scattering properties of fraction of an acidhydrolyzed dextran. Dextran Specifications: Label (Ex/Em): Fluorescein (494/521) Size: 40,000 MW. The Transdermal diffusion cell system was protected from light. Ahn, G.Y., Eo, HS., Kim, D. et al. Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. Microporation was performed 2 times on each sample. The micropores were developed on pig skin and human cadaver skin with dermal and epidermal layers by the microporation device. 1994, 95-0355. Progress in Measurement, Interpretation and Application to Structure in Dilute Solution. Pharm Res. Microneedles and their applications. Although, in literature there is much information on hydrodynamic measurements from determinations viscosity; very few of them evaluate the situation at different temperatures. 2012;101(8):28619. (2003). Pharm Res. The important role of thermodynamic interaction parameter in the determination of theta temperature, dextran/water system. Tirtaatmadja, V.; Dunstan, D. E.; Boger, D. V. (2001). Increased TEWL values are evidence of micropores formation. Gillespie, T.; Hulme, M. A. TEWL measurements were taken to assess the integrity of the skin barrier and the extent of SC disruption. Chen Rong Huei; Wei Yu Chen; Shang Ta Wang; Chu Hsi Hsu; Min Lang Tsai (2009). The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. Nordmeier, Eckliard (1993). Microporations were performed 2, 5, and 10 times and the skin damage was confirmed using the TEWL measurement. The optical image and histological analysis confirmed that the micropores on the skin were recovered over time. Format), Citation-(BibTeX Dextran is soluble in water, methyl sulphoxide, formamide, ethylene glycol, glycerol, 4-methylmorpholine-4-oxide, and hexamethyl phosphamide [4]. princess alice of battenberg cause of death, anthony abetemarco brother, antique hiatt handcuffs,